Connectivity analysis assumes that there is only one foreground particle and no cavities in the image. Purify locates all particles in 3D and removes all but the largest foreground and background particles, as a filtering step to prepare images for Connectivity. It is a specialised implementation of the particle-finding methods in Analyse Particles, which are designed to reduce recursion and make full use of system resources.

Odgaard A, Gundersen HJG (1993) Quantification of connectivity in cancellous bone, with special emphasis on 3-D reconstructions. Bone 14: 173-182. doi:10.1016/8756-3282(93)90245-6.

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